Product Introduction
HopCellTM Human Cortical Neurons differentiated from HopCellTM Human Neural Progenitor Cells are cultures of excitatory (70-80%) and inhibitory (20-30%) cortical neurons that closely resemble neuronal networks in vivo. These neurons form networks (clustered firing) starting from 14 days after inducing differentiation, express all 6 layers of cortical markers, are ≥98% MAP2+ and can be maintained for more than 3 years in vitro.
Immuno-fluorescent Photomicrographs of HopCellTM Human Cortical Neurons
Primary human cortical neurons are populations representative of the six cortical layers with both excitatory glutaminergic neurons (about 70-80%) and GABAergic interneurons (about 20-30%, subtypes of inhibitory neurons within, for example PV, nNOS, SST).
Electrophysiology Activities ( DIV14 )
Spontaneous and synchronous firing of HopCellTM Human Cortical Neuron recorded by multielectrode array (MEA)
Product Information
Applications
Applications
1、Neurotoxicity assays using HopCellTM Human Cortical Neuron（DIV13） were measured with microplate spectrophotometer（384-well plates）
2、MK801 protects HopCellTM Human Cortical Neurons（DIV68） against Oxygen-Glucose Deprivation-induced cell death
On DIV (Days in vitro) 68, HopCell Human
Cortical Neurons were treated with Oxygen Glucose Deprivation (OGD) to mimic
cerebral ischemia in vitro, and the cell viability was detected by
measuring the expression level of CCK8. The remarkable excitotoxicity was induced in human cortical neurons after OGD
treatment. On the other hand, neurotoxicity was markedly attenuated by the NMDA
receptor antagonist MK801, which added to the culture medium during the OGD
treatment. MK801 significantly protected the Human Cortical Neurons against
excitotoxicity caused by OGD. This
experiment demonstrated that Human Cortical Neurons can be used to mimic
cerebral ischemia in vitro.
3、Neurite outgrowth assay using HopCellTM Human Cortical Neuron（DIV1） were observed with the Operetta® High Content Imaging System
4、Spontaneous and and synchronous firing of HopCellTM Human Cortical Neuron recorded by multielectrode array (MEA)